I am facing a problem on loading the aqueous plant extract on SDS PAGE, every thing was done as per the protocol. ON the same the methanol extract got loaded , but the aqueous extract spills out of the well
Don´t you have glycerol in your sample buffer? That should prevent any spilling, as long as you´re loading the gel quite slowly.
Priyankar Dey: Thats what Protein of Methanolic extract, who uses petrochemical seperation for SDS Page
Hello
I do not know about the extraction method
You should talk to the chemist
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