Does anybody have the protocol for the estimation of resveratrol by spec method?

Raghavendra Sathyanarayana @Raghavendra_Sathyanarayana

05 May 2014 1 2K Report

Djanna Fadul Cornago

You may try the method by Camont et al. I have attached the reference article here.

Badges
Science topic

Similar topics
Chemistry
Biochemistry

More Raghavendra Sathyanarayana's questions See All

What should be the mobile phase for the crude plant extract?

The methanolic crude plant extract is showing antibacterial activity and even methanol to water from 10% to 70% ratio. But when the crude extract loaded on a silica column , we are failing to get...

02 March 2019 1,052 6 View

How to lode aqueous plant extract on SDS PAGE?

I am facing a problem on loading the aqueous plant extract on SDS PAGE, every thing was done as per the protocol. ON the same the methanol extract got loaded , but the aqueous extract spills out...

31 December 2018 1,506 3 View

How to identify insulin standard by SDS page ?

I would like to know the presence of Insulin mimicking peptide from plant sources, Preliminary I am trying to confirm it by running SDS PAGE. But I am not able to run the Insulin standard. What...

05 June 2018 5,619 5 View

If we have a plant extract, extracted by buffers for secondary metabolite assay what type of column chromatography can we go for?

Initially we extracted with alcohol it is showing anti microbial activity but its not stable, hence we used buffer of pH 4, 7 and 9. Now the zone of inhibition is stable and for further...

11 December 2017 3,512 11 View

How to isolate insulin mimicking peptide from the callus cultures ?

We have a plant where in the literature says it has insulin mimicking peptide. So we established callus from the leaf and the stem. Now we have to go further to identify the presence of that...

11 December 2017 8,630 1 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

How can I add a new WSN routing protocol in NS2 or NS3?

I'm trying to find in literature a way to help me adding a modified Yen's algorithm in ns2 or its variance ns3 or another simulator. Have you some tips which can be helpful, I'm opening in the...

19 February 2021 7,981 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View