05 May 2014 1 2K Report

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More Raghavendra Sathyanarayana's questions See All
What should be the mobile phase for the crude plant extract?

The methanolic crude plant extract is showing antibacterial activity and even methanol to water from 10% to 70% ratio. But when the crude extract loaded on a silica column , we are failing to get...

02 March 2019 1,116 6 View

How to lode aqueous plant extract on SDS PAGE?

I am facing a problem on loading the aqueous plant extract on SDS PAGE, every thing was done as per the protocol. ON the same the methanol extract got loaded , but the aqueous extract spills out...

31 December 2018 1,569 3 View

How to identify insulin standard by SDS page ?

I would like to know the presence of Insulin mimicking peptide from plant sources, Preliminary I am trying to confirm it by running SDS PAGE. But I am not able to run the Insulin standard. What...

05 June 2018 5,723 5 View

How to isolate insulin mimicking peptide from the callus cultures ?

We have a plant where in the literature says it has insulin mimicking peptide. So we established callus from the leaf and the stem. Now we have to go further to identify the presence of that...

11 December 2017 8,691 1 View

If we have a plant extract, extracted by buffers for secondary metabolite assay what type of column chromatography can we go for?

Initially we extracted with alcohol it is showing anti microbial activity but its not stable, hence we used buffer of pH 4, 7 and 9. Now the zone of inhibition is stable and for further...

11 December 2017 3,578 11 View

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