Has anyone tried MBP (MAB386) free floating in rat brain (40um) sections?

I have fixed rat brain sections, 40um thick, cut on cryostat. The anti myelin antibody, MAB386 gives me non-specific staining in free floating but works beautifully on paraffin sections. Any...

10 October 2018 2,479 2 View

How I register my research paper and need to know citation no and h-Index ?

For NAAC I need to register my research papers in Research gate and need to know citation no and h-Index

16 December 2017 9,524 7 View

How to calculate the extended Acm line and alpha/alpha+gamma line below eutectoid temperature in Fe-Fe3C diagram (low alloy steel -C-0.2,Mn-0.8,Si0.2?

I want to calculate the equilibrium pearlite, ferrite and bainite phase fraction below eutectoid temperature using the extended Acm and alpha/alpha and gamma line

15 October 2017 2,985 3 View

Is there any reason why the MapReduce program execution time taken on Single VM is much more than the mapreduce program run on mini-cloud ?

I have created a mini-cloud of Hadoop in my lab on few machines, when I executed a mapreduce job on the mini-cloud and on single VM(independent, not in cloud) , i found that the time taken on...

03 March 2017 7,876 5 View

How to characterise Different pencil graphite elctrodes (5H,4H,3H,2H,H, HB, B,2B,3B,4B,5B ) .

I want to test pencils of different grades (i.e different carbon content H,2H,3H,4H,5H...B,2B,3B,4B,5B...AND HB) as electrodes. The OCP which I got is not following trend. Can anybody help in this...

29 July 2016 7,350 2 View

Why is there the differences in the genotype band intensities in gel electrophoresis . can anyone please explain ?

10 April 2016 3,786 3 View

From where I may get MO3.13, HOG cell line of oligodendrocytes?

Is there any better option than these cell lines to study oligodendrocytes in vitro? I need to create hyperglycemic and hypoglycemic condition. Then I aim to inhibit perticular protein by adding...

21 October 2015 2,204 2 View

Can any one tell me about how to learn neuronal cell culture? is there any place in india where I can get training? i

Basically I want to learn neuronal cell culture

20 June 2014 1,795 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

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02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View