Has anyone tried MBP (MAB386) free floating in rat brain (40um) sections?

I have fixed rat brain sections, 40um thick, cut on cryostat. The anti myelin antibody, MAB386 gives me non-specific staining in free floating but works beautifully on paraffin sections. Any...

10 October 2018 2,479 2 View

How I register my research paper and need to know citation no and h-Index ?

For NAAC I need to register my research papers in Research gate and need to know citation no and h-Index

16 December 2017 9,524 7 View

How to calculate the extended Acm line and alpha/alpha+gamma line below eutectoid temperature in Fe-Fe3C diagram (low alloy steel -C-0.2,Mn-0.8,Si0.2?

I want to calculate the equilibrium pearlite, ferrite and bainite phase fraction below eutectoid temperature using the extended Acm and alpha/alpha and gamma line

15 October 2017 2,985 3 View

Is there any reason why the MapReduce program execution time taken on Single VM is much more than the mapreduce program run on mini-cloud ?

I have created a mini-cloud of Hadoop in my lab on few machines, when I executed a mapreduce job on the mini-cloud and on single VM(independent, not in cloud) , i found that the time taken on...

03 March 2017 7,876 5 View

How to label DNA with YOYO-1?

I would like to look at the internalization of DNA-YOYO1 by one of my peptides in some cell lines. In this regard, I am looking for a detailed protocol for plasmid DNA labeling with YOYO-1...

14 November 2016 418 2 View

How to characterise Different pencil graphite elctrodes (5H,4H,3H,2H,H, HB, B,2B,3B,4B,5B ) .

I want to test pencils of different grades (i.e different carbon content H,2H,3H,4H,5H...B,2B,3B,4B,5B...AND HB) as electrodes. The OCP which I got is not following trend. Can anybody help in this...

29 July 2016 7,350 2 View

Why is there the differences in the genotype band intensities in gel electrophoresis . can anyone please explain ?

10 April 2016 3,786 3 View

From where I may get MO3.13, HOG cell line of oligodendrocytes?

Is there any better option than these cell lines to study oligodendrocytes in vitro? I need to create hyperglycemic and hypoglycemic condition. Then I aim to inhibit perticular protein by adding...

21 October 2015 2,204 2 View

Can any one tell me about how to learn neuronal cell culture? is there any place in india where I can get training? i

Basically I want to learn neuronal cell culture

20 June 2014 1,795 5 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Exit velocity of natural gas from pressurized tank?

Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...

26 February 2021 6,253 3 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

Any interests in participating to an experiment in space?

Hello, The UN Office of Outer Space Affairs has a program called access to space for all that enables institutions to have hands on expertise related to access to space. There are three elements...

22 February 2021 7,980 3 View

Thermal and mechanical properties of concrete and steel after cooling?

How do the thermal conductivity, specific heat capacity, plastic strain, critical compressive displacement, and thermal expansion of both hot-rolled steel and concrete change after cooling based...

15 February 2021 1,385 5 View

How to modify micro-structure of Pure Copper which is available as a flat plate of 25 X 25 sq. cm?

Hi everyone, for a thesis project I have to modify the micro-structural properties of pure Cu flat plate of dimension 25 X 25 sq. cm . As I tried searching the available literature, I found a lot...

09 February 2021 5,090 3 View

What is the most efficient way to generate amorphous silica in LAMMPS?

I would like to generate multiple large samples of amorphous silica in LAMMPS. Currently I am generating it by beginning with a lattice of silicon and oxygen atoms and using a melting and...

28 January 2021 6,128 4 View

Is it possible to economically reuse the heat content of water vapour that is generated during quenching of continuous casting of steel?

A huge amount of water is evaporated to cool down and solidify the liquid metal strands coming out of bottom of CCM mould. The steam contains significant latent and sensible heat energy, besides...

26 January 2021 3,446 4 View