How I register my research paper and need to know citation no and h-Index ?

For NAAC I need to register my research papers in Research gate and need to know citation no and h-Index

16 December 2017 9,524 7 View

How to calculate the extended Acm line and alpha/alpha+gamma line below eutectoid temperature in Fe-Fe3C diagram (low alloy steel -C-0.2,Mn-0.8,Si0.2?

I want to calculate the equilibrium pearlite, ferrite and bainite phase fraction below eutectoid temperature using the extended Acm and alpha/alpha and gamma line

15 October 2017 2,985 3 View

Is there any reason why the MapReduce program execution time taken on Single VM is much more than the mapreduce program run on mini-cloud ?

I have created a mini-cloud of Hadoop in my lab on few machines, when I executed a mapreduce job on the mini-cloud and on single VM(independent, not in cloud) , i found that the time taken on...

03 March 2017 7,876 5 View

How to label DNA with YOYO-1?

I would like to look at the internalization of DNA-YOYO1 by one of my peptides in some cell lines. In this regard, I am looking for a detailed protocol for plasmid DNA labeling with YOYO-1...

14 November 2016 418 2 View

How to characterise Different pencil graphite elctrodes (5H,4H,3H,2H,H, HB, B,2B,3B,4B,5B ) .

I want to test pencils of different grades (i.e different carbon content H,2H,3H,4H,5H...B,2B,3B,4B,5B...AND HB) as electrodes. The OCP which I got is not following trend. Can anybody help in this...

29 July 2016 7,350 2 View

Why is there the differences in the genotype band intensities in gel electrophoresis . can anyone please explain ?

10 April 2016 3,786 3 View

From where I may get MO3.13, HOG cell line of oligodendrocytes?

Is there any better option than these cell lines to study oligodendrocytes in vitro? I need to create hyperglycemic and hypoglycemic condition. Then I aim to inhibit perticular protein by adding...

21 October 2015 2,204 2 View

Can any one tell me about how to learn neuronal cell culture? is there any place in india where I can get training? i

Basically I want to learn neuronal cell culture

20 June 2014 1,795 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View