How to increase 3T3-L1 adipocyte differentiation rate?

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Tengku Faris

Cant even reach 50% lipid droplet formed

Venil N Sumantran

Do you mean that 50% of the cells get lipid drops ? That is quite good for 3T3L1 cells. The initial plating density, and the level of confluence when you added these factors can affect the differentiation rate. Hope this helps!

Yes, but seems i need more of it. Should i increase insulin concentration up to 20 ug/ml?

Denise Cooper

It is also going to depend on the source of the 3T#-L1 cells. Is this a low passage? The source of insulin is also important.

Annie Macpherson

Use Rosiglitazone as shown here

http://www.ncbi.nlm.nih.gov/pubmed/22425542