How to quantify and differentiate the amount of DNA and RNA fluorescence stained with DAPI?

09 October 2016 3,532 4 View

Different protein size due to species variation?

06 July 2016 4,562 4 View

Is there any difference on primary antibody binding region?

31 December 2015 6,256 2 View

Why can't Dragendorff's reagent precipitate caffeine?

See above

04 May 2014 7,321 1 View

Are there any differences between Wistar and Sprague-Dawley rats?

Can anyone tell me any differences in term of their behaviour, physical appearance, habit, fur, eyes etc. and may suggest better for antidiabetic research?

04 May 2014 5,647 1 View

What would be correct way to remove chlorophyll from leaves extract?

working on ethanolic extract of plant leaves and appears very dark colour. how can I ensure that the chlorophyll wont interrupt with my compounds in extract?

03 April 2014 8,066 3 View

Is there any difference between orthophosphoric and phosphoric acid?

While running HPLC I have prepared 0.1% (v/v) orthophosphoric acid diluted in water, as mobile phase. Does anyone know how can I prepare this?

31 December 2013 7,556 9 View

DMSO wont dilute my pure compound. How to solve this?

Testing against cancer cells, I couldn't seem to dissolve my sample, even in DMSO. A suspension was formed. My preparation method: initially 1ug sample was dissolved in 10ul DMSO and further mixed...

31 December 2013 5,938 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View