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Questions related from Tengku Faris
I am staining cells with dapi, which thought to bind A-T rich region of double helix. and we know that dapi fluorescence intensity increases when attached to DNA as compared to unbound...
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I'm measuring protein expression across human embryonic stem (ES) cell (as control), differentiated ES cell and E15 fetal mouse tissue (presumably same stage as differentiated ES cell) by western...
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Could anyone please advice is there any difference ordering antibodies which immunogen mapped to either C-, N-terminus, or mid-region? Which region is preferable?
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Can anyone tell me any differences in term of their behaviour, physical appearance, habit, fur, eyes etc. and may suggest better for antidiabetic research?
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See above
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working on ethanolic extract of plant leaves and appears very dark colour. how can I ensure that the chlorophyll wont interrupt with my compounds in extract?
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Testing against cancer cells, I couldn't seem to dissolve my sample, even in DMSO. A suspension was formed. My preparation method: initially 1ug sample was dissolved in 10ul DMSO and further mixed...
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While running HPLC I have prepared 0.1% (v/v) orthophosphoric acid diluted in water, as mobile phase. Does anyone know how can I prepare this?
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I've tried with Insulin:10ug/ml, IBMX: 0.5mM, Dexamethasone: 1.0uM
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