Hi all,
I am searching for kits (apart from MicroSeq, PCR) or other simple techniques to identify at least the class of fungi. Any ideas?
Thank you in advance,
Sofya
Hi... Dr. Sofya Pozdniakova ...
After isolation and purification the fungus, shoud be study of morphological characteristics such as (colony form, color, colony background) and microscopic characteristics such as ( mycelium, shape of conidia, spores ), as well lab tests such as (Hair Perforation test for Dermatophytes fungi), or directly way DNA-sequence, to be able to confirm identity.
Good luck.
before doing a molecular identification you can start with a morphological identification.
you can identify the genus from the morphological study: the color of the mycelium, presence the septum in the filament or not, existence of sporocyst and endogenous spore, phialide and conidium. the size and shape of the conidia. Conidiogenesis is an important key for the identification of sporulating fungi.
Hi... Dr. Sofya Pozdniakova ...
After isolation and purification the fungus, shoud be study of morphological characteristics such as (colony form, color, colony background) and microscopic characteristics such as ( mycelium, shape of conidia, spores ), as well lab tests such as (Hair Perforation test for Dermatophytes fungi), or directly way DNA-sequence, to be able to confirm identity.
Good luck.
You can ascertain species identity for microbes by employing the polyphasic approach (PA). This is a good briefer you can refer to: https://www.sciencedirect.com/science/article/pii/S0167701214001353
Using PA, you will need morphological, molecular, and biochemical data and use all of these in speculating the possible identity of your fungal species.
Hi everyone, for complete identification of a certain species simple steps should be followed from morphological level to genetic, and for each there are many ways in which atlas,chemical test, PCR kits are so useful.
Dear colleagues, thank you very much for the comprehensive recommendations. Sofya.
Dear, Sofya .. you must begin with morphological study , you should isolate the fungi and purify them and see the morphological features, then prepare slides with lactophinol and see the microscopic characters of the fungi . Finally you should confirm the identification with PCR.
Good luck
Dear Sofya,
Please have a look at these useful links.
Book Molecular Identification of Fungi
http://www.ascofrance.fr/uploads/forum_file/Humber-RA-Chapter-V-Entomofungi-identification-0001.PDF
https://www.researchgate.net/post/What_is_the_best_method_for_the_identification_of_Fungus_under_microscope
https://www.researchgate.net/post/What_are_the_basic_methods_to_identify_fungal_isolates
https://www.researchgate.net/post/How_can_I_identify_fungi_species_level_from_agar_plates
https://www.researchgate.net/post/Identification_of_fungus
https://www.researchgate.net/post/How_to_identify_and_characterize_fungi_growing_on_PDA
https://www.researchgate.net/post/What_are_the_different_method_for_Identify_unknown_fungi
https://www.researchgate.net/post/How_can_I_identify_endophytic_fungi
https://www.researchgate.net/post/Identification_of_fungal_strain
Good luck!
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