Difference between Polycarbonate and quartz filter?

Dear colleagues, I have just started to work on the environmental project. I have to analyze the microorganisms in the air. For that I have to choose the correct type of filters. In my group, 2...

17 June 2018 4,697 3 View

How to identify fungi?

Hi all, I am searching for kits (apart from MicroSeq, PCR) or other simple techniques to identify at least the class of fungi. Any ideas? Thank you in advance, Sofya

03 June 2018 1,143 9 View

How do I do live immunostaining and sorting of cryopreserved monocytes?

i would like to do surface immunostaining and live sorting of cryopreserved CD14+ human monocytes for further experiments. Does anybody have experience with this kind of experiments? Do I need to...

12 February 2016 1,782 5 View

How can I improve transfection in H9C2 cells?

17 May 2015 4,583 6 View

Does someone see a difference in the morphology of H9C2 cells during culturing?

I am working with H9C2 cell line and I see difference in morphology of this cells during the culturing. They can be round as well as prolonged ones and it seems to me that the round shape appears...

14 December 2014 7,098 4 View

With H9C2 how can I check the differentiation of the cell line?

H9C2 cell line can differentiate in cardiac or skeletal cells. I have to show that my cells are definitely cardiac ones. Does anyone have experience with this?

08 April 2014 5,420 4 View

CGMP ELISA based assays

I am trying to measure cGMP in H9C2 cells. The data I get are out of the calibration curve. Does anyone has an experience or idea how this could be improved? I already increased cell number and it...

01 January 1970 7,121 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View