How effectively extract DNA from quartz filter and liquid?

Good evening, I am working on the establishment of DNA extraction from quartz filter and collection liquid that were used for air sampling. The idea is to extract as much as possible DNA of high...

03 February 2020 4,323 2 View

Difference between Polycarbonate and quartz filter?

Dear colleagues, I have just started to work on the environmental project. I have to analyze the microorganisms in the air. For that I have to choose the correct type of filters. In my group, 2...

17 June 2018 4,697 3 View

How to identify fungi?

Hi all, I am searching for kits (apart from MicroSeq, PCR) or other simple techniques to identify at least the class of fungi. Any ideas? Thank you in advance, Sofya

03 June 2018 1,143 9 View

How do I do live immunostaining and sorting of cryopreserved monocytes?

i would like to do surface immunostaining and live sorting of cryopreserved CD14+ human monocytes for further experiments. Does anybody have experience with this kind of experiments? Do I need to...

12 February 2016 1,782 5 View

How can I improve transfection in H9C2 cells?

17 May 2015 4,583 6 View

Does someone see a difference in the morphology of H9C2 cells during culturing?

I am working with H9C2 cell line and I see difference in morphology of this cells during the culturing. They can be round as well as prolonged ones and it seems to me that the round shape appears...

14 December 2014 7,098 4 View

With H9C2 how can I check the differentiation of the cell line?

H9C2 cell line can differentiate in cardiac or skeletal cells. I have to show that my cells are definitely cardiac ones. Does anyone have experience with this?

08 April 2014 5,420 4 View

CGMP ELISA based assays

I am trying to measure cGMP in H9C2 cells. The data I get are out of the calibration curve. Does anyone has an experience or idea how this could be improved? I already increased cell number and it...

01 January 1970 7,121 2 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Which index or sites are reliable in determining of plant species?

There are many indexes when determining plant species: Plants of the World Online (POWO), International Plants Names Index (www.ipni.org), The Plant List (www.theplantlist.org) and others. But...

26 February 2021 3,404 10 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How do historians determine the credibility sources in modern history?

For a source to be considered reliable it must contain accurate historical information. But how does one determine the accuracy and reliability of sources in modern and recent history since not...

23 February 2021 1,346 13 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

Can i quantify protein concentration in sds page?

I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?

22 February 2021 215 2 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View