14 Questions 11 Answers 0 Followers
Questions related from Sofya Pozdniakova
I am culturing microorganisms from the air (collected by Coriolis sampler in liquid base) as a criteria to obtain the most adequate sampling conditions. The aim is to find the sampling condition...
04 March 2021 455 3 View
Good evening, I am working on the establishment of DNA extraction from quartz filter and collection liquid that were used for air sampling. The idea is to extract as much as possible DNA of high...
04 February 2020 4,436 2 View
Dear Colleagues, I am searching for the adequate manner to count bacterial colonies. So far I have tried ImageJ and OpenCFU and was not much satisfied - both softwares count the background as...
02 April 2019 7,246 0 View
Dear colleagues, I have just started to work on the environmental project. I have to analyze the microorganisms in the air. For that I have to choose the correct type of filters. In my group, 2...
18 June 2018 4,802 3 View
Hi all, I am searching for kits (apart from MicroSeq, PCR) or other simple techniques to identify at least the class of fungi. Any ideas? Thank you in advance, Sofya
04 June 2018 1,228 9 View
Hi, I just started Seahorse experiments and I am confused with the fact that I should change the medium for assay medium 1 hour before measurements. Meaning there are changes in pH, as far as this...
23 March 2016 1,374 3 View
I transfected H9C2 cells with different transfection reagents such as Lipofictamine 2000 and 3000, Viromer yellow and red. The best option for me was Viromer red. At the beginning the...
18 May 2015 4,673 6 View
I do an overexpression of the estrogen receptors in the H9C2 cell line and then treat the cells with estradiol and then measure luciferase assay. But there is no response in luciferase activity....
27 March 2015 8,118 2 View
I am working with H9C2 cell line and I see difference in morphology of this cells during the culturing. They can be round as well as prolonged ones and it seems to me that the round shape appears...
15 December 2014 7,194 4 View
I isolate mitochondria from mice tissue, as marker of the mitochondrial outer membrane I use TOM 40 and I have unspecific staining.
01 December 2014 4,727 3 View
H9C2 cell line can differentiate in cardiac or skeletal cells. I have to show that my cells are definitely cardiac ones. Does anyone have experience with this?
09 April 2014 5,526 4 View
Which one is better to use? What are the advantages of choosing one over another?
15 February 2014 7,206 1 View
I am truying to isolate intact mitochondria from cells using teflon homogenizer. I am using the proteinase K as a marker of intact inner mitochondrial membrane but I can't manage with that ( I...
05 February 2014 8,133 35 View
I am trying to measure cGMP in H9C2 cells. The data I get are out of the calibration curve. Does anyone has an experience or idea how this could be improved? I already increased cell number and it...
01 January 1970 7,208 2 View
