Curcumin (368.385 g/mol).
DMSO purity 99.99%
Curcumin solubility in DMSO (25 mg/ml),
I need to create a stock concentration of 40mM. How much of each do I need? Can someone please explain how this is calculated? Thank you
368.385 g of curcumin dissolved in 1 liter gives a 1 M solution. To get a 0.04 M solution (which is uequivalent to 40 mM) you multiply 0.04 X 368.385 = 14.7354 g; this is the amount you would need to dissolve in 1 liter. If you want less than 1 liter you need to reduce the amount used. For example if you want 100 ml, you would dissolve 1.47 g of curcumin. Remember to dissolve in less than 100 ml of DMSO (use 90 ml) and make up to 100 ml after dissolving. If you need 1 ml, you would dissolve 14.7 mg of curcumin.
The curcumin solubility data lets you know that this stock can be made (25 mg/ml is equivalent to 25 g per liter; therefore the 14.7 g of curcumin per liter you need for a 40 mM solution is achievable).
368.385 g of curcumin dissolved in 1 liter gives a 1 M solution. To get a 0.04 M solution (which is uequivalent to 40 mM) you multiply 0.04 X 368.385 = 14.7354 g; this is the amount you would need to dissolve in 1 liter. If you want less than 1 liter you need to reduce the amount used. For example if you want 100 ml, you would dissolve 1.47 g of curcumin. Remember to dissolve in less than 100 ml of DMSO (use 90 ml) and make up to 100 ml after dissolving. If you need 1 ml, you would dissolve 14.7 mg of curcumin.
The curcumin solubility data lets you know that this stock can be made (25 mg/ml is equivalent to 25 g per liter; therefore the 14.7 g of curcumin per liter you need for a 40 mM solution is achievable).
Vincent Mulholland
It turns out that not everything is done for money.
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