How many naïve T cells we can get from a C57BL/6J mouse spleen and lymph nodes? or in other word, what percentage of the cells from spleen and lymph nodes are naïve T cells?
The percentage of naïve T cells in the spleen and lymph nodes of a C57BL/6J mouse can vary depending on various factors such as age, sex, immune status, etc. However, it is generally accepted that in healthy adult mice, the percentage of naïve T cells in the lymphoid organs is around 70%.
As far as I know a typical mouse spleen contains 80 to 100 million spleenocytes and T cells comprise roughly 25% of the total splenocyte population. How it is possible that naïve T cells constitue 70% of the cells!! I want to know how many of the 25% T cells in spleen are naïve T cells?!
If you want to have an idea for absolute numbers and distribution, please check our paper on T cell development
Article A role for the histone H2A deubiquitinase MYSM1 in maintenan...
We used C57BL/6J littermate controls in our experiments. Moreover, a FACS staining for CD62L and CD44 in the CD4/ CD8 compartment, with CD44 low CD62L high expressing T cells generally considered to be naive.
Please check Fig. 3 of the paper for more details and the materials and methods for the experimental protocol.