I used 2.4D 1 ppm to my culture, but it's not friable one. i have to get friable texture for my cell suspension (Elicitor applied). Anyone can help me? just share :). maybe we can discuss together if you have same problem or same research
To obtain friable callus you should avoid using high concentrations of 2-4,D; instead, I suggest applying TZD and removal of any ammonia form of nitrogen from culture medium.