How to get a good yield of RNA from neutrophils?

More Débora Barbosa Vendramini-Costa's questions See All

Could anyone tell me any molecular biology company that sequences mitochondrial genome?

I'm looking for a company to sequence the complete mitochondrial genome of some species of fish.

14 December 2020 3,154 3 View

How to know if 87Sr/86Sr is reliable for Sr isotope chemostratigraphy in marbles?

I am currently looking for parameters to know if 87Sr/86Sr data is reliable for chemostratigrahy in marbles, and/or parameters to know if those marbles have had some post-depositional...

30 November 2020 9,032 8 View

Can my RNA interference have any influence in my endougenos control gene?

Good night all. I'm using RNAi to knockdown a transcription factor from a primary cell and I need to validate by westernblot. With Ponceau marking i can see that i'm putting the same amount of...

25 August 2020 263 3 View

Piecewise multiplicative scatter correction in R?

Anyone is aware of an R package available online to perform a Piecewise Multiplicative Scatter Correction (PMSC)? This technique is an advance of the Multiplicative Scatter Correction (MSC)...

23 August 2020 2,893 2 View

How can i model a shock tube with 2 different gases on Fluent?

Hi, I'm currently trying to study shock tube analysis on fluent and I have modelled a shock tube with a driver section (high-pressure) and a driven section (low pressure). What I want to study is...

22 July 2020 8,162 3 View

Can someone suggest an image analysis process to evaluate student performance in drawing a removable partial denture structure on a dental cast?

The evaluation of a removable partial denture structure drawing on a cast is an important part the student of dental prosthodontics learning experience. This a cumbersome situation for faculty...

27 March 2020 6,953 6 View

Is it possible to compare the means of climatic data of different time periods?

I have two different datasets of air temperature measured in two periods of time and I would like to ask if it is possible the comparison between their means. It is like to compare day and night...

05 March 2020 2,896 3 View

How to classify labeled data?

I have unlabeled data that contains a hundred fields, and it is necessary to label it. One way to do it is to interpret the data and create new data that could be labeled, or find out some of...

26 February 2020 1,602 2 View

What is the best method for mammalian blood RNA extraction?

Hi everyone, Does anyone have protocol for mammalian blood RNA extraction with Trizol? I have not obtained good results. Thanks

02 September 2019 2,722 3 View

Teacher-centered or student-centered approach for children with learning difficulties?

I can't find any evidence in favor of Teacher-centered or Student-centered approach for children with learning difficulties. DO you know about published studies?

21 May 2019 4,539 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Pranav Joshi

Hello,

Try other RNA isolation kit such as RNeasy mini kit from Qiagen. It works.

Hope this helps.

Alexander Sasha Vlassov

MirVana (spin column based) or MagMax (magnetic bead based) kits work really well

Kay-Dietrich Wagner

Use Trizol or if your cell pellet is not really dry Trizol LS, add Glycogen to the isopropanol precipitation step.