Débora Barbosa Vendramini-Costa

3 Questions 5 Answers 0 Followers

Questions related from Débora Barbosa Vendramini-Costa

How to get a good yield of RNA from neutrophils?

Hello! I am getting very low concentration of RNA from murine neutrophils, even from 1 million cells. Is there any tip to improve the concentration? I disrupt the pellet with syringes and I am...

06 June 2016 9,612 3 View

Does any of you have experience inducing ER stress in murine bone marrow monocytes and neutrophils?

I am trying to establish the ideal conditions to stimulate ER stress in monocytes and neutrophils from murine Bone marrow. Which would be the right dose and time of treatment with Thapsigargin? I...

02 February 2016 8,917 0 View

Does anyone know of a reliable NRF2 antibody?

Which band do you look at (100 kDa, 68 kDa)? Hi, We are currently using Santa Cruz anti-NRF-2 antibody to detect NRF-2 expression. We see bands usually at 65, 68 kDa. However, sometimes a band...

03 March 2015 9,103 4 View