Dear colleague,
I am testing on formulation of cationic lipid nanoparticles. The lipid compostiin is DOTAP: DSPC: Cholesterol: PEG2000 DSPE=50: 10: 39: 1. The buffers of 50 mM sodium acetate at pH 4 and 1XPBS, containing 0.9% NaCl, at pH 7 are used as the aqueous cargos. Could I have precious advice about how to reduce lipid nanoparticle size down to 100 nm (80-120 nm) with PDI less than 2. Thank you.
Sincerely,
Jacky
Dear Jacky,
Do you pass your NP trough an extrusion filter under high pressure after you add the acques phase to your lipidic film? I believe this is kinda necessary to ensure you have small particles at the end. You will have to start from a 400nm filter and slowly go down (untill 100nm)....the more you pass trough the filters, the lower will be the pdi (you want it lower than 0.2, not 2). You could also try using a 0.5% of PEG rather than 1 and maybe lower the Dotap to 20-40%. Another option is sonication (which is usually done before extrusion). Another option is a centrifugation, to remove the bigger size NP formed (12000rpm for 10min should be sufficient). Then take the supernatant and check with DLS.
I hope this helps
Regards
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