Hi all!
I have found specific mutations in dengue genome sequences apparently related to the infection outcome. Here my questions:
How can I find if this mutation is synonymous or non-synonymous?
How can I verify if this mutation affects immunogenic/biologic properties of the protein?
I have some ideas but wondering if there's a standardised way to do this.
Kind regards,
B
First part is easy. Go to GenBank and open a genome for your virus and then check if your mutation is within a known protein by nucleotide position.
If your mutation is within a protein paste the genomic region including the mutation into Expasy to get the translation for each frame. You can then do an amino acid sequence alignment using an online tool like MUSCLE against the reference amino acid sequence from GenBank.
The checking for changes in immunogenicity or function is the hard part.
- Badges
- Science method