How to extract the protein from the SDS-PAGE gel ?

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Rajaei Almohammed

Hi Venkata,

You will have to do an in-gel digest, there are many protocols online. This is a very common protocol to prepare samples for mass spec. It involves a de staining steps as well.

Good luck!

Adam B Shapiro

Here is a reference for the method:

http://www.nature.com/nprot/journal/v1/n6/full/nprot.2006.468.html

SOUMYA RANJAN JENA

in gel digestion(destaining with ammonium bicarbonate and acetonitrile,dehydration with acetonitrile,reduction with DTT,alkylation with iodoacetamide ,dehydration with acetonitile).

Raja Shekhar Reddy

You have to do an in-gel digest this mainly used for mass spectrometric characterization of proteins and proteomes