Hello to everyone
I am working in the field of stress physiology, I am comparing different parameters between Male animals Vs Female animals and within Male and female animals, each group contains three different time duration of stress. For this, I have to do two-way ANOVA in the SPSS (version 20) software. would you please explain to me how to do two-way for in this case.
Methods
I am giving stess to animal during the gestational period with three different trimester first, second and third trimester.
Example 1: for example i am calculating body weight gain during gestational period 1-10 days and 11-22 days gestational period for all stress exposed groups and a control groups.
Example 2 : I am doing Neurobehavioural analysis (anxiety behaviour) for offspring both sex male and female of three stress exposed group and a control group.
how to do two-way ANOVA for these cases and how to enter the data in the datasheet. In these cases which i have to take as dependent variable, independent variable and fixed factor.
Here is the sample data in the data sheet. please help me out
Thanks in advance for your valuable suggestion.
Actually both data formats long and wide is applicable to repeated measures analysis by SPSS. But for beginners I recommend wide data format in which repeated measures are represented on different columns side by side.
As far as I understand from your post, in Example 1: you have 1 within subject factor (time) and 1 between subjects factor (group). You have weight gain in different periods, you need to enter them in different columns. Since you have also a group variable it will hold another column. So for this dataset you will have 3 columns for repeated measures and one column for group variable. Data structure will be GRP, WGAIN1,WGAIN2,WGAIN3.
In Example 2: you have 2 between subjects factors (gender and group). You need one column for gender, one column for group and one column for outcome, the dependent variable (the measure you want to test). You need to put dependent variable into Dep Variable box in GLM->Univariate procedure, and the other two factors into Fixed factors box (since they are fixed). There is no dataset attached.
There is an ambiguity about the stress exposed groups you mentioned. Are they repeatedly measured thrice? Or are there three different groups?
Hello Mehmet,
Thanks for your response. Its three different groups.
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