How to quantify the homocysteine levels in the brain tissue, please give me procedure for estimation of homocysteine..
Hello,
I used an adjusted HPLC protocol, described by Pfeiffer.
Maybe these references can help you:
Pfeiffer CM, Huff DL, Gunter EW. Rapid and accurate HPLC assay for plasma
total homocysteine and cysteine in a clinical laboratory setting. Clin Chem. 1999
Feb;45(2):290-2. PubMed PMID: 9931056.
de Rezende MM, D'Almeida V. Central and systemic responses to
methionine-induced hyperhomocysteinemia in mice. PLoS One. 2014 Aug
25;9(8):e105704. doi: 10.1371/journal.pone.0105704. eCollection 2014. PubMed
PMID: 25153079; PubMed Central PMCID: PMC4143291.
Sincerely,
Marina M de Rezende.
How to estimate the Homocysteine levels in Brain??. Available from: https://www.researchgate.net/post/How_to_estimate_the_Homocysteine_levels_in_Brain#55102239cf57d78f4b8b46da [accessed Mar 23, 2015].
Thank you so much madam for your help...
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