How to do the calculation for buffer preparation from scratch?

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Why is the DPPH activity low in leaf than in root while on the other hand the TFC and TPC high in leaf than in root of Withania somnifera?

Why is the DPPH activity low in leaf than in root while on the other hand the TFC and TPC being high in leaf than in root of Withania somnifera?

13 May 2023 8,507 3 View

Two way ANOVA using missing data in Minitab?

The effect of different growth stages stage 1, 2 3 and 4 and different concentrations of cadmium (0 ppm, 40 ppm, 80 ppm, 120 ppm, 160 ppm and 200 ppm) has to be checked on protein accumulation....

28 February 2023 1,352 4 View

Interpretation of results of Duncan's multiple range test (post hoc) for two way ANOVA in SPSS?

1. How to interpret the results of Duncan's multiple range test for two way ANOVA in SPSS? 2. Do we assign alphabets to represent Duncan's value like the way we do in post hoc test for one way...

22 February 2023 8,076 0 View

Will there be any differences in the Raman shift values of a material when we change laser wavelengths?

Please provide the references for the same

14 July 2021 557 3 View

How can we do mechanistic analysis of concrete pavements using software?

concrete containing waste materials which also made of OPC 15, my doubt here is any possibility there to analyze using software with experimental results?

08 June 2021 1,839 4 View

Any one know how to do simulation of concrete pavements?

i need software foe the simulation of concrete paements and also analysis

07 May 2021 6,263 6 View

What is an alternative for 0.45 µm nylon membrane filter?

The sample which I am working on requires filtration using 0.45 µm nylon membrane filter for HPLC analysis. However, it is costly. Could someone provide an alternative for this? Or can I skip the...

30 April 2021 6,373 3 View

Is it safe to isolate important metabolites from medicinal plants grown in metal contaminated soil?

Is it safe to isolate important metabolites from medicinal plants grown in metal contaminated soil? I am checking the effect of metals on plant growth, morphology biochemical variation, and...

24 March 2021 8,373 5 View

A protocol for reagent preparation for catalase, ascorbate peroxidase and superoxide dismutase assay?

I am working on the effect of heavy metals on plants. However, to check the activity of antioxidant enzymes I need to prepare reagents in bulk. Buying an assay kit is not affordable. Can someone...

21 March 2021 3,995 3 View

Why it is important to study effect of heavy metal in medicinal plants?

07 March 2021 3,175 5 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

What is the best method for removing paraffin from plant samples prepared for microtome?

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24 July 2024 3,087 3 View