Akshatha Banadka

13 Questions 28 Answers 0 Followers

Questions related from Akshatha Banadka

Two way ANOVA using missing data in Minitab?

The effect of different growth stages stage 1, 2 3 and 4 and different concentrations of cadmium (0 ppm, 40 ppm, 80 ppm, 120 ppm, 160 ppm and 200 ppm) has to be checked on protein accumulation....

01 March 2023 1,304 4 View

What is an alternative for 0.45 µm nylon membrane filter?

The sample which I am working on requires filtration using 0.45 µm nylon membrane filter for HPLC analysis. However, it is costly. Could someone provide an alternative for this? Or can I skip the...

01 May 2021 6,301 3 View

How to do the calculation for buffer preparation from scratch?

I want to prepare 10 mM phosphate buffer of pH 7.6 using potassium dihydrogen phosphate and dipotassium monohydrogen phosphate. Kindly someone help me with the detailed calculation?

14 April 2021 6,656 2 View

A protocol for reagent preparation for catalase, ascorbate peroxidase and superoxide dismutase assay?

I am working on the effect of heavy metals on plants. However, to check the activity of antioxidant enzymes I need to prepare reagents in bulk. Buying an assay kit is not affordable. Can someone...

22 March 2021 3,950 3 View

How to manually calculate p value in ANOVA? what is the equation or formula for it?

04 March 2021 7,969 5 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

02 March 2021 7,293 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

24 February 2021 3,333 7 View

Best solvent for L-DOPA extraction and HPLC analysis?

I'm working on Mucuna pruriens and want to quantify L-DOPA. Could someone please suggest the cheap and best solvent for the extraction and the solvent system that has to be used for its...

21 February 2021 6,284 2 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

12 February 2021 6,894 6 View

Simultaneously performing biochemical tests

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 January 1970 7,208 6 View

A detailed explanation for the calculation of proline by Bates et al. method?

Could someone give a stepwise detailed explanation for calculation of proline by Bates et al. method?

01 January 1970 1,196 4 View

Why is the DPPH activity low in leaf than in root while on the other hand the TFC and TPC high in leaf than in root of Withania somnifera?

Why is the DPPH activity low in the leaf than in the root, while on the other hand, the TFC and TPC are high in the leaf than in the root of Withania somnifera? The DPPH activity is expected to...

01 January 1970 6,246 7 View

Two way ANOVA using missing data

The effect of different growth stages stage 1, 2 3 and 4 and different concentrations of cadmium (0 ppm, 40 ppm, 80 ppm, 120 ppm, 160 ppm and 200 ppm) has to be checked on protein accumulation....

01 January 1970 3,827 20 View