The sample which I am working on requires filtration using 0.45 µm nylon membrane filter for HPLC analysis. However, it is costly. Could someone provide an alternative for this? Or can I skip the membrane filtration (I will be quantifying L-DOPA)
Hi Akshatha,
Filtering your sample is always a good idea depending on the type of sample... What type of sample you are analysing (dialysate, homogenate, other: plasma, urine, blood?) In case of dialysates, these are pretty clean samples in general because the dialysis probe usually 'acts' a an good filter. What sample prep are you performing besides filtering? and what type of LC analysis you are conducting (HPLC-ECD, HPLC-?). Another good precaution is using a guard column or pre filter in front of your analytical column (so in case you have clogging, you only have to replace your guard).
Regards, Hendrik
You could use centrifugation, but please share some details to make a good assessment
Hendrik-Jan Brouwer sir, Thank you for your response:
This is the protocol that I will be following:
The sample is a plant. I will be using methanol and HCl as the solvent followed by centrifugation. The final step demands nylon membrane filtration(0.45 microns)
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