Hello,
I want to use Tamoxifen to induce cre-knockout mice
in Jacson lab, they mentioned to dissolve tamoxifen in corn oil at a concentration of 20 mg/ml by shaking overnight at 37°C,
https://www.jax.org/research-and-faculty/tools/cre-repository/tamoxifen
However, in other protocols it is mentioned to add ethanol as well to dissolve tamoxifen.
My question is it amust to use ethanol to disolve tamoxifen? or I can just dissolve it in oil overnight without using ethanol ?
and if I must use ethanol, is there any other alternatives for sonication or vaccum heating ?
Thank u in advance
1. Place 5 ml of corn oil in scintillation vial.
2. Heat corn oil in 42° C for 30 min. In the meantime, take the Tamoxifen out and
equilibrate at RT before weighing.
3. Add 100 mg Tamoxifen into the pre-heated 5 ml corn oil (20 mg/ml final
concentration).
4. Wrap vial with aluminium foil.
5. Place on a rocker at 37° C for several hours.
6. If Tamoxifen is not going into solution you can vortex frequently and break clumps
using a 5ml syringe with 18 G needle.
7. Place at 4° C - good for up to one month.
- Badges
- Science topic