02 February 2018 2 3K Report

Hello guys,

I am running western for the 3rd time for the same protein. First two times I can see clear one band. It is inducible protein.

However, on the 3rd time, I have two bands , instead of one band, and no induction (The same two bands in both ctrl and stimulated sample),

the only different thing I made in the 3rd time is that I used less concentration dose to stimulate my gene of interest.

Anyone knows why I see two bands instead of one? anyone experienced this issue before ?

I added an image for my data to further make my question clear.

Thank you.

Doaa

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