Why shRNA control cell line behave like that ?

I have KO cancer cell line, and I am applying shRNA to target some genes. My control is RFP-shRNA. I ran western blot to check my protein of interest, and i have found that KO cell line with...

08 September 2018 5,117 6 View

Why flow cytometry and Qpcr are different ?

Hello everyone, I have unexplained phenomenon in my data regarding one cytokine. The level of this cytokine by Qpcr is very high in knockout more than WT mice in naive condition and after...

08 September 2018 4,417 3 View

What is this bacteria ? how to get rid of it ?

Dear bacteria and cell culture specialists, Recently we encountered this bacteria during cell culture, please see the attached images. These bacteria doesn't change the PH of the culture media...

03 April 2018 2,510 4 View

Can I use sonication in EMSA?

Hello, I'm doing EMSA using adherent cell line . I extracted the nucleus part but it was very viscous. Why the nuclear extract was very viscous ? And can I apply sonication ? Thank you.

03 April 2018 8,852 4 View

Why DNA migrates slower than DNA/protein complex ?

Hello fellows, I am doing EMSA. I wanted to check for the binding and efficacy of DNA and nuclear extract proteins. So, I run Acrylamide gel (native, 5% , 0.5xTBE buffer, with DNA view dye )....

03 April 2018 7,945 23 View

How to find a promoter sequence for specific gene ?

Hello fellows, How can I find a promoter sequence for a certain gene ? ( simple way to explain , i don't have much bioinformatic background) Thanks in advance

02 March 2018 9,567 11 View

Double band in western blot ?

Hello guys, I am running western for the 3rd time for the same protein. First two times I can see clear one band. It is inducible protein. However, on the 3rd time, I have two bands , instead...

01 February 2018 2,616 2 View

How to troubleshoot isotype control for flow cytometry ?

Dear fellows, I am facing a problem using Isotype control, for example, when I run flow , the signal in un-stimulated samples is 10%, and in stimulated samples is 12%. However, the isotype...

10 November 2017 8,393 2 View

Flag-tag on C-terminal or N-terminal?

Hello Fellows, I want to clone a protein with a Flag but I can't decide where should I place the Tag on C-terminal or N-terminal since some papers put it on N-terminal but others put on...

10 November 2017 1,280 4 View

How to measure Calcium ?

07 August 2017 948 6 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View