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Questions related from Ivan Luzardo-Ocampo
Dear all, I would like to quantify protein nitrosylation but most ELISA kits are very expensive. I have already quantified the amount of nitrites in cell cultures using the Griess Reaction. Is...
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Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...
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Dear all. Is there a way to simulate temperature or voltage treatments to a single protein? If so, is there an online web server to conduct it or using BioVia Discovery/AutoDock tools? Thanks in...
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Dear all, we are evaluating the cytotoxic effect of plant extracts on HT-29 and HCT116 colon cancer cells. We have determined the IC50 values for each cell line, however, we want also to test...
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Hello, I'll perform flow cytometry (Caspase-1, Annexin-V, PI assay) in differentiated macrophages (from THP-1 monocytes to macrophages using PMA). I usually detach the cells using TryPLE and wait...
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Hi, I am trying to dissolve pure reagent grade Quercetin into cell culture medium (DMEM+sodium bicarbonate+antibiotics) but it clearly does not form a solution. I've found that DMSO might serve...
23 July 2018 3,931 3 View
Does HPLC grade reagents serve for UHPLC-MS quantification? Even filtered with 0.22 micron pore? Thanks in advance
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Dear all...I'm evaluating inflammation on macrophages (cell line: RAW 264.7) but actually I'm just culturing them. Is it possible to detach them using trypsin? I've read in some articles that...
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