Ivan Luzardo-Ocampo

10 Questions 12 Answers 0 Followers

Questions related from Ivan Luzardo-Ocampo

Nitric oxide in tissues as indirect measurement of protein nitrosylation?

Dear all, I would like to quantify protein nitrosylation but most ELISA kits are very expensive. I have already quantified the amount of nitrites in cell cultures using the Griess Reaction. Is...

04 November 2021 1,189 1 View

Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

17 February 2021 3,371 3 View

Temperature/voltage simulations of a protein?

Dear all. Is there a way to simulate temperature or voltage treatments to a single protein? If so, is there an online web server to conduct it or using BioVia Discovery/AutoDock tools? Thanks in...

15 June 2020 1,275 3 View

Which should be the concentration of extracts to evaluate in normal colon cells?

Dear all, we are evaluating the cytotoxic effect of plant extracts on HT-29 and HCT116 colon cancer cells. We have determined the IC50 values for each cell line, however, we want also to test...

11 October 2019 9,403 3 View

Flow cytometry in TryPLE detached macrophages?

Hello, I'll perform flow cytometry (Caspase-1, Annexin-V, PI assay) in differentiated macrophages (from THP-1 monocytes to macrophages using PMA). I usually detach the cells using TryPLE and wait...

24 May 2019 4,962 3 View

How to dissolve reagent grade Quercetin into cell culture medium?

Hi, I am trying to dissolve pure reagent grade Quercetin into cell culture medium (DMEM+sodium bicarbonate+antibiotics) but it clearly does not form a solution. I've found that DMSO might serve...

23 July 2018 3,947 3 View

HPLC grade reagents for UHPLC-MS ?

Does HPLC grade reagents serve for UHPLC-MS quantification? Even filtered with 0.22 micron pore? Thanks in advance

04 February 2018 1,801 7 View

Cell viability increases by MTT

Hi, I haven't found and explanation for cell viability increase when RAW 264.7 macrophages are stimulated with LPS (compared with RAW 264.7 macrophages with no stimulation of LPS). Regarding this,...

04 June 2017 157 3 View

DMEM Contaminated Media or floculation of components?

Dear all. I need to know if the picture showed represent some sort of contamination or just floculation of any components? It corresponds to DMEM Media + 1.5 g/L Sodium Bicarbonate + 10 mL Sodium...

08 February 2017 8,569 3 View

Detaching RAW 264.7 Macrophages using Trypsin?

Dear all...I'm evaluating inflammation on macrophages (cell line: RAW 264.7) but actually I'm just culturing them. Is it possible to detach them using trypsin? I've read in some articles that...

28 May 2016 6,725 3 View