Any manual method for sepsis DNA extraction?

Hi. I am looking for manual method for sepsis DNA extraction. No kit based method required. Can you please suggest some good protocols? I want DNA extraction of both, cfDNA and bacterial cell DNA.

08 September 2018 5,664 0 View

Bacteria DNA/ cell free DNA extraction from blood plasma?

How to extract free floating Bacterial DNA or cell free DNA from blood plasma? I am looking for non-kit based rapid protocols.

07 August 2018 4,582 3 View

Different number of peaks for similar qPCR program?

It was observed that with same samples and same primers, one qPCR reaction will show multiple peaks, but the second reaction (plate) will have only one Tm peak. Why is it so?

02 March 2018 2,035 5 View

Removal of phenols while performing nucleic acid extraction from tea and cloves samples

07 August 2017 1,909 1 View

I want to validate my Enzyme activity data using bioinformatics tools. Any suggestions regarding which bioinfo tools can be used for the same?

I have done enzyme activity assay of mitochondrial enzymes. I want to validate this data using bioinformatics tools. Are there any tools which can be used for performing in silico enzyme activity...

08 September 2016 1,502 4 View

Determining protein samples?

Are there any other methods other than gel electrophoresis to determine if protein has been extracted out of the samples?

08 September 2016 193 6 View

What is Rouging?

What is the exact mechanism of Rouging in agriculture?

01 February 2015 8,326 4 View

Softwares for phylogenetic tree?

Can you please suggest good softwares for preparing both rooted and unrooted phylogenetic trees?

01 January 1970 7,732 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View