Bacteria DNA/ cell free DNA extraction from blood plasma?

How to extract free floating Bacterial DNA or cell free DNA from blood plasma? I am looking for non-kit based rapid protocols.

07 August 2018 4,582 3 View

Different number of peaks for similar qPCR program?

It was observed that with same samples and same primers, one qPCR reaction will show multiple peaks, but the second reaction (plate) will have only one Tm peak. Why is it so?

02 March 2018 2,035 5 View

Removal of phenols while performing nucleic acid extraction from tea and cloves samples

07 August 2017 1,909 1 View

I want to validate my Enzyme activity data using bioinformatics tools. Any suggestions regarding which bioinfo tools can be used for the same?

I have done enzyme activity assay of mitochondrial enzymes. I want to validate this data using bioinformatics tools. Are there any tools which can be used for performing in silico enzyme activity...

08 September 2016 1,502 4 View

Determining protein samples?

Are there any other methods other than gel electrophoresis to determine if protein has been extracted out of the samples?

08 September 2016 193 6 View

How to differentiate between the different types (isoforms) of Casein through PAGE?

What protocols are available to distinguish between different forms of milk casein?

05 June 2016 4,528 3 View

What is Rouging?

What is the exact mechanism of Rouging in agriculture?

01 February 2015 8,326 4 View

Softwares for phylogenetic tree?

Can you please suggest good softwares for preparing both rooted and unrooted phylogenetic trees?

01 January 1970 7,732 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View