Hi. I am looking for manual method for sepsis DNA extraction. No kit based method required. Can you please suggest some good protocols? I want DNA extraction of both, cfDNA and bacterial cell DNA.
How to extract free floating Bacterial DNA or cell free DNA from blood plasma? I am looking for non-kit based rapid protocols.
07 August 2018 4,705 3 View
It was observed that with same samples and same primers, one qPCR reaction will show multiple peaks, but the second reaction (plate) will have only one Tm peak. Why is it so?
02 March 2018 2,116 5 View
Hi all. When I am trying to extract DNA or RNA from Tea and cloves, high amount of phenol are present in the extract. Even repeated washes with Lithium chloride is not solving the problem. Can...
07 August 2017 2,016 1 View
I have done enzyme activity assay of mitochondrial enzymes. I want to validate this data using bioinformatics tools. Are there any tools which can be used for performing in silico enzyme activity...
08 September 2016 1,604 4 View
Are there any other methods other than gel electrophoresis to determine if protein has been extracted out of the samples?
08 September 2016 294 6 View
What protocols are available to distinguish between different forms of milk casein?
05 June 2016 4,627 3 View
What is the exact mechanism of Rouging in agriculture?
01 February 2015 8,436 4 View
Can you please suggest good softwares for preparing both rooted and unrooted phylogenetic trees?
01 January 1970 7,827 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View