Any manual method for sepsis DNA extraction?

Hi. I am looking for manual method for sepsis DNA extraction. No kit based method required. Can you please suggest some good protocols? I want DNA extraction of both, cfDNA and bacterial cell DNA.

08 September 2018 5,664 0 View

Bacteria DNA/ cell free DNA extraction from blood plasma?

How to extract free floating Bacterial DNA or cell free DNA from blood plasma? I am looking for non-kit based rapid protocols.

07 August 2018 4,582 3 View

Different number of peaks for similar qPCR program?

It was observed that with same samples and same primers, one qPCR reaction will show multiple peaks, but the second reaction (plate) will have only one Tm peak. Why is it so?

02 March 2018 2,035 5 View

Removal of phenols while performing nucleic acid extraction from tea and cloves samples

07 August 2017 1,909 1 View

I want to validate my Enzyme activity data using bioinformatics tools. Any suggestions regarding which bioinfo tools can be used for the same?

I have done enzyme activity assay of mitochondrial enzymes. I want to validate this data using bioinformatics tools. Are there any tools which can be used for performing in silico enzyme activity...

08 September 2016 1,502 4 View

Determining protein samples?

Are there any other methods other than gel electrophoresis to determine if protein has been extracted out of the samples?

08 September 2016 193 6 View

How to differentiate between the different types (isoforms) of Casein through PAGE?

What protocols are available to distinguish between different forms of milk casein?

05 June 2016 4,528 3 View

Softwares for phylogenetic tree?

Can you please suggest good softwares for preparing both rooted and unrooted phylogenetic trees?

01 January 1970 7,732 0 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View

Where can I obtain agricultural land use data for Wales, UK?

The Gower peninsula around Swansea is my target area

16 February 2021 7,473 3 View

How to differentiate the economic productivity in agriculture and in manufacturing sector?

Most of the references I found were on the economic productivity in agriculture but very limited application in manufacturing sector. I would appreciate if anyone could share the references on...

15 February 2021 8,342 7 View

What are the ways to test the knowledge level of agricultural labourer about their day to day work?

what kind of test can I do for testing the level of knowledge about their daily operation?

03 February 2021 7,583 10 View

What do you think about spatially based sustainable agriculture?

I am currently researching sustainable agriculture. I am interested in elaborating the LCA method and spatial mapping analysis. Most of the sustainable agricultural research only looks at 3...

17 January 2021 6,201 6 View

Which LCIA method is most suitable for assessing the impacts of nitrate leaching in groundwater (eutrophication )?

The eutrophication of groundwater by nitrate leaching is major environmental problem in Germany, especially in areas with intensive agriculture and high N-fertilizer inputs. In addition there are...

16 January 2021 5,182 7 View

Can you suggest literature studying the conditions information and evidence can influence manager decisions in social impact sectors?

I am working on improving the use of digital innovations at scale globally. Most of the innovations I work with provide relevant knowledge on improving the performance of projects, programs, and...

16 January 2021 1,120 5 View

What type of plants is better to work in gene regulation control project ?

I Interested in to work in gene network modeling thus I need to know what is future of gene network modeling in agriculture thanks

04 January 2021 6,527 3 View

No title

02 January 2021 7,172 4 View

Can you suggest Standard vocabulary for characterizing research and development initiatives (project,program,policy,networks) and their performance?

I am doing text mining on the performance on research for development interventions in low income countries. I need some standard dictionaries and typologies to map the content of the paper in a...

31 December 2020 5,329 4 View