Greetings everyone!

I am a Biology undergraduate student and I am doing my thesis on the bioremediation capabilities of fungi in the recent Oriental Mindoro, Philippines oil spill. With that I would like to test for the presence of oil-degrading fungi in the sand. I am planning to have 3 sampling sites (2 oil-contaminated areas, and 1 control) with 3 samples each. With that, may I know how I can determine which oil-contaminated areas to sample? Is there a minimum distance between the 2 sites that I should take note of?

Thank you!

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