How to determine Optimal Cut-off in Disease Identification by using Graphpad prism?

More Lina Naji's questions See All

The importance of humanistic care?

Humanistic care is mainly about caring for people's living conditions, affirming people's living conditions and dignity, and pursuing human freedom and liberation. With the continuous development...

10 July 2024 5,107 2 View

My EMSA Blot is not sharp and blurry after exposure with a Phosphorimager (Typhoon). What can I do to fix that?

I'm currently trying to do some EMSA using P32. The first two gels looked perfect, with sharp bands and no background. However, all the following ones looked like the blot below. I'm not doing...

18 June 2024 1,967 2 View

Can sample size differ across paths in a path analysis?

Hello there! I'm looking at a mediation model with 2 predictors, 2 mediators and 3 outcomes. My full sample size is 558, but one of those outcomes only has data for 410 participants. So when I...

18 May 2024 1,404 4 View

Authenticity of "Plasma Science and Technology" Journal?

Dear colleagues, I require urgent assistance regarding the authenticity of the "Plasma Science and Technology" journal. Upon investigation, I found two websites bearing the same name and ISSN...

17 April 2024 5,732 2 View

How can I prepare a sample of green coffee extract obtained by supercritics to be analyzed by HPLC?

I would like to know how I can prepare my sample of supercritical extract of green coffee beans if I want to analyze chlorogenic acid and I need to prepare the sample to analyze it by HPLC. I...

31 March 2024 854 2 View

I tried to precipitate my Protein with using iced acetone left it in ice but there is no pellet ?

I used the iced acetone in -20C , used 4 times double the volume of my protein, and incubated the mixture inside the ice for more than 3 hours and I didn't notice any pellet.

22 February 2024 7,424 0 View

What is this structure in colorectal cancer cell line?

Hi, I could see this morphology under the microscope (black arrow, 20x). Could someone identify what this morphology/ structure could be? and if so how to stain it with a specific dye? Cells are...

21 February 2024 9,450 4 View

How can I seed 0.5x10^6 cells per well in a 96-well plate?

How can I seed 0.5x10^6 cells per well in a 96-well plate (I only need to seed 5 wells)? I have a T25 flask (5 mL total volume) with a cell density of 0.6x10^6 cells/mL.

17 February 2024 5,535 1 View

What should I do if my polyacrylamide gels have this stain?

In this electrophoresis , i'm using serum samples without any treatment, mixing them with sample buffer in a 1:1 ratio. Te run is performed at 150v for 1 Hour, an this is the result after...

14 November 2023 2,732 5 View

What is the best research topic for integration of AI ( DL) with lean six sigma in order to improve operational control?

I will be using it for DBA thesis

04 August 2023 8,237 1 View

Standard curve of H2O2?

Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...

26 July 2024 3,459 2 View

Can I use soybean, sunflower, or olive oil to prepare a standard curve for lipid estimation in plants or pollen?

I am trying to analyze lipids in plants; specifically pollen by using Sulfo-Phospho-Vanillin (SPV) Method! Can I prepare a standard curve for this estimation using soybean, sunflower, or olive...

01 July 2024 3,266 1 View

Generating multiple standard curves from a single template?

Hi everyone! After thinking about it on my own, I'm posting a question here. Has anyone ever tried creating qPCR standard curve samples (plasmid or DNA standard) by inserting multiple target...

30 June 2024 1,569 1 View

About the standard of the FRAP in calculation?

Good morning/day/evening salaam I’d like to ask about the FRAP assay again. I have read some articles which represent the data of frap assay as mg trolox equivalent/L, mg ascorbic acid...

27 June 2024 5,239 0 View

can I use Roc Curve in a pilot study?

Hi everyone, we know that the analysis of pilot studies should be based on descriptive statistics and ideally not involve any inferential statistics (i.e., P values). Based on this can I use Roc...

24 June 2024 4,402 7 View

How to increase qPCR efficiency to acceptable range?

My aim is to create a standard for qPCR for absolute quantification of DNA extracted from soil. The reaction contains PowerUp SYBR Green Master Mix, BSA, primer pair (A189F-MB661R- 0.67 uM), and...

10 June 2024 6,708 9 View

XPSpeak41 after optimizing the graph is still not well fit?

Hi, i am currentyl fitting mno2 material into the peak at Mn 2p 3/2 and Mn 2p 1/2. in the right side of the peak, the tip highlighted in red is still not fit enough. is there any idea how to fit...

05 June 2024 7,957 6 View

What is Area Under the Curve for detecting SARS-COV-2 neutralizing antibodies?

I have a question about AUC for calculating SARS-CoV-2 neutralizing antibody tests. AUC here is not the Area Under the ROC Curve. After six serial dilutions of human serum, an indirect ELISA was...

05 June 2024 8,191 0 View

How can I determine the cut off from the ROC curve?

Hello, If I have the ROC curve from SPSS output, How can I determine the cut off?

01 June 2024 9,670 3 View

Complex dynamic systems theory data analysis inquiry?

How many participants and times are required to conduct latent growth-curve modeling or cross-lagged panel analysis. Thanks in advance.

29 May 2024 1,853 1 View

David Eugene Booth

What do you mean by optimal? I assume you have an ROC curve. How did you get the ROC curve? What is CAD disease? Best wishes David Booth

Lina Naji

Dear David Eugene Booth thank you for your reply

CAD Stands for coronary artery disease

and I want a method that " how Graphpad specify the Cut-off value " for me

regards

Thanks very much for your reply. I believe the attached Google search should answer all of your questions. Best wishes David Booth

David Eugene Booth thank you

appreciate it

I found this pdf and it was helpful