I am wondering if somebody could assist/tell me on the methods how to determine the HPV DNA and its onchoproteins/mRNA from women having full blown cervical cancer.
Dear RG users, I am looking for reflection and an experience from anyone working on either QuantiVirus ® HPV E6/E7 mRNA assay or HPV E6/E7 mRNA RT-qPCR using cervical sample. Similarity and...
10 November 2018 1,103 0 View
I am looking for a reflection from expertise on the specific advantage and challenges of qRT-PCR of the E6/E7 mRNA test of HR-HPVs over the the APTIMA ® HPV Assay.
08 September 2018 4,001 2 View
Dear RG users, Is there a methodological difference between scoping review and systematic review? What is the clear boundary of these review types?
06 July 2018 8,735 10 View
According to the latest publication on nature (https://www.nature.com/subjects/diagnostic-markers), a diagnostic marker is defines as 'a biological parameter that aid the diagnosis of a disease'....
05 June 2018 6,559 0 View
SPSS and Stata are apparently useful for epidemiological studies despite personal preferences. What is really the basic difference between these two packages? Which one is more convenient and...
04 May 2018 703 0 View
I usually use EndNote to mange citations. Recently I have learned that Mendeley, Zotero and Reference manager tools are also widely applicable in the scientific world. I need experience from...
03 April 2018 4,206 0 View
I have learned from different literature is that the origin of viruses is not well known though there are different theories on the subject. I am looking explanations from RG users on current...
02 March 2018 2,738 5 View
WHO’s first release of surveillance data on antibiotic resistance reveals high levels of resistance to a number of serious bacterial infections in both high- and low-income countries. Though...
01 February 2018 4,609 8 View
What are the most important tips that we should consider to find suitable journal for our paper?
01 February 2018 3,308 8 View
Dear RG users, I am wondering suggestions from colleagues; what is the clear boundary between OR and RR? How can I easily understand and interprate these epidemiological tools? Reflections are...
31 December 2017 9,927 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View