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10 November 2018 916 0 View

What is the advantage of E6/E7 mRNA qRT-PCR assay for HR-HPVs over APTIMA HPV Assay in cervcal cancer screening?

08 September 2018 3,857 2 View

Systematic review Vs Scoping review?

Dear RG users, Is there a methodological difference between scoping review and systematic review? What is the clear boundary of these review types?

06 July 2018 8,423 10 View

Factors to be considered while working with a diagnostic marker ?

According to the latest publication on nature (https://www.nature.com/subjects/diagnostic-markers), a diagnostic marker is defines as 'a biological parameter that aid the diagnosis of a disease'....

05 June 2018 6,404 0 View

SPSS vs Stata?

SPSS and Stata are apparently useful for epidemiological studies despite personal preferences. What is really the basic difference between these two packages? Which one is more convenient and...

04 May 2018 532 0 View

Which type of citation manager is use friendly and freely available?

I usually use EndNote to mange citations. Recently I have learned that Mendeley, Zotero and Reference manager tools are also widely applicable in the scientific world. I need experience from...

03 April 2018 4,053 0 View

How viruses evolved?

I have learned from different literature is that the origin of viruses is not well known though there are different theories on the subject. I am looking explanations from RG users on current...

02 March 2018 2,576 5 View

High levels of antibiotic resistance found worldwide, new data shows. How this could be contained?

01 February 2018 4,463 8 View

How can we find a suitable journal to publish our research paper?

What are the most important tips that we should consider to find suitable journal for our paper?

01 February 2018 3,113 8 View

What is the meaning of Odds ratio, Risk ratio and Risk difference and how they are interprated in epidemiological studies?

Dear RG users, I am wondering suggestions from colleagues; what is the clear boundary between OR and RR? How can I easily understand and interprate these epidemiological tools? Reflections are...

31 December 2017 9,723 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View