I am looking for a reflection from expertise on the specific advantage and challenges of qRT-PCR of the E6/E7 mRNA test of HR-HPVs over the the APTIMA ® HPV Assay.
Hi dear Awoke,
These is no big differnces between both of these techniques, despite each one has its own limitations.
Can you find the links below, please?
1- https://jcm.asm.org/content/49/2/557
2- https://www.hologic.com/sites/default/files/package-insert/AW-14517-001_003_01.pdf
Best wishes
Sarmad
Dear Sarmad,
Thank you!
10 November 2018 916 0 View
Dear RG users, Is there a methodological difference between scoping review and systematic review? What is the clear boundary of these review types?
06 July 2018 8,423 10 View
According to the latest publication on nature (https://www.nature.com/subjects/diagnostic-markers), a diagnostic marker is defines as 'a biological parameter that aid the diagnosis of a disease'....
05 June 2018 6,404 0 View
SPSS and Stata are apparently useful for epidemiological studies despite personal preferences. What is really the basic difference between these two packages? Which one is more convenient and...
04 May 2018 532 0 View
I usually use EndNote to mange citations. Recently I have learned that Mendeley, Zotero and Reference manager tools are also widely applicable in the scientific world. I need experience from...
03 April 2018 4,053 0 View
I have learned from different literature is that the origin of viruses is not well known though there are different theories on the subject. I am looking explanations from RG users on current...
02 March 2018 2,576 5 View
01 February 2018 4,463 8 View
What are the most important tips that we should consider to find suitable journal for our paper?
01 February 2018 3,113 8 View
Dear RG users, I am wondering suggestions from colleagues; what is the clear boundary between OR and RR? How can I easily understand and interprate these epidemiological tools? Reflections are...
31 December 2017 9,723 2 View
11 December 2017 193 4 View
Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...
03 March 2021 3,731 3 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
Is there a powerful system for the security of the systems distributed on IoT systems?
02 March 2021 3,858 10 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...
01 March 2021 2,272 4 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View