How to design best primers to amplify a region in mice genomic DNA?

More Lakshani Perera's questions See All

Does anyone have experience in using AVL simulation software specifically AVL Cruise M?

currently I am trying to model and engine system where i want to simulate it using Cruise M and then to test it with different fuels to observe the behavior of said engine. I am having issues with...

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How to Recognize Different Emotions Using Heart Rate Variability Signal Features?

For research purposes, I need to determine how citizens perceive space through user experience; to do so, I need to identify different emotions using Heart Rate Variability Signals or ECG data. I...

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What is the importance of cleaning the site with 70% alcohol before taking a skin scraping, especially from the soggy and purulent lesions??

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What are the types of leaves which the outer skin ( epidermis) transparent to mid infrared frequencies?

Most of the leaves reflect mid and far infrared radiation. But there may be few types that their outer layer is transparent to mid or far infrared.

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Is there a Sub-ambient radiative cooling paint or coating available commercially?

There are many white coatings or solar coatings available commercially, but is there a radiative cooling paint available commercially?

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Can biophysical/ optical properties of leaves could be modified by genetic engineering?

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Access and administration of the revised (KMPAI)?

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Crystal Defects in Zeolites?

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How to obtain an equation for the optimized model in ANN?

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How to quantify elements using narrow scans in CasaXPS?

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Does crude extraction using NaOH and Tris work well with Fungi?

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Anyone having idea about VN primer for miRNA primer design ?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

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How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

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Abhijeet Singh

Genomes for selected organisms (primary reference assembly only)

Frederic Lepretre

Hi Lakshani,

Md Abdur Rahim send you a good way to test your primers once you'll get the sequence from the same website (http://genome-euro.ucsc.edu). choose your species and target and you'll arrive at the right place to download the genomic sequence.

fred