How to design best primers to amplify a region in mice genomic DNA?

More Lakshani Perera's questions See All

Why there are multiple spikes around 50Hz (Power Interference) in frequency domain (fft)?

I am working on a project related to sEMG classification. In order to process the signal, I am trying to filter the signal, especially power interference. The signal is acquired from Delsys...

07 December 2020 9,745 10 View

What is the best way to plot Ct values for a gene expression in different Samples ?

Hi I want to plot a graph for Ct values from qPCR assay that resulted from a gene expression in different samples.

29 October 2020 1,384 3 View

How to filter resonance frequencies in Matlab?

Following Image presents the frequency domain of sEMG signal which was sampled at 4000Hz. However, unexpected noises were captured during the experiment. I believe that these are some sort of...

29 August 2020 7,421 3 View

What changers can occur if Arsenobetaine (Organoarsenic) digested with an acid (Nitric acid) ?

Arsenobetaine is the non toxin organic form of Arsenic commonly found in fish.If muscle of fish samples subject to a acid digestion, will non toxic nature be changed

30 May 2020 9,109 4 View

Any explanation about the effect of the pH value on SARS-CoV-2 Virus activity ?

Some studies reveal that SARS-CoV-2 virus activity can be mitigated at the alkaline pH medium (above pH= 7.5). Any explanation about the mechanism or the Chemistry behind this phenomenon.

28 May 2020 9,352 3 View

How to input a set of data from an Excel sheet into MATLAB Simulink?

I've got Solar irradiance data and Temperature data for a month in 1 hour time intervals in a Excel sheet. I've modelled a Simulink block set to get the Output of a PV array. Unfortunately, I'm...

27 May 2020 3,072 5 View

What is the best technique / extraction method for extract sesquiterpene coumarins from oligo-gum resins of Ferula asafoetida ?

Phyto-chemicals such as Conferone, Samarcadin, Bdrakemin Farnesiferol A, Fernesiferol C and Galbanic acid can be isolated from Ferula asafoetida. suggest proper extraction methods and solvent to...

22 May 2020 6,350 6 View

Can the sign of a coefficient change from + to - when treat for endogeneity?

Hi, I'm running a model to see the growth effects of the exchange rate. The coefficient that I got for the real effective exchange rate is positive significant under the fixed effect model and...

19 November 2019 6,677 1 View

How do I amplify a 7.4 KB fragment in mouse genomic DNA ?

I am struggling to amplify an 7.4 kB fragment which I need to confirm my CRISPR KO. I have tried Pfu and Phusion polymerase, used both HF and GC buffers, tried 50 - 70 C temp gradient, DMSO...

27 March 2019 9,699 3 View

Can anyone recommend a method for ethylene gas detection in an air sample?

I am looking for a method to detect ethylene gas concentration in a green house. I have tried SPME , Head space and GC-MS analysis but results were not satisfied. So is it better to detect...

10 January 2019 7,832 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Abhijeet Singh

Genomes for selected organisms (primary reference assembly only)

Frederic Lepretre

Hi Lakshani,

Md Abdur Rahim send you a good way to test your primers once you'll get the sequence from the same website (http://genome-euro.ucsc.edu). choose your species and target and you'll arrive at the right place to download the genomic sequence.

fred