HI all,
I need to access revised (KMPAI) and use it for my dissertation for my MSc. Any advice on how to go about doing that? I've messaged Dr. Kenny already.
As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...
27 February 2021 2,595 8 View
Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...
23 February 2021 7,429 1 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
17 February 2021 4,844 4 View
Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...
16 February 2021 2,377 7 View
Hi, I'm trying to merge files from a data base of 20 files, each containing about 4000 subjects. Unfortunately the ID numbers are actually a combination of numbers and letters (e.g., b1458219)....
15 February 2021 5,833 3 View
Good Evening, My name is David Marshall and I am Doctor of Management Student at Colorado Technical University Online. I just recently received approval from the IRB to start data collection on...
08 February 2021 346 7 View
I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...
08 February 2021 2,673 1 View
Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...
07 February 2021 6,973 3 View
Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...
07 February 2021 3,643 4 View
Hello, In my sandwich-type ELISA which is testing for small molecule inhibition of a protein-protein interaction, it seems that DMSO percentage and dilution protocol are important. I...
31 January 2021 6,771 1 View
Hello, everyone! I have chemically synthesized antioxidant peptide isolated from the plant. I have to validate its bioactivities in Adult zebrafish. I have to administer this peptide to zebra...
01 February 2021 5,809 3 View
Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...
30 January 2021 2,865 4 View
I came across a recent taxonomic work, where two genera were synonymized. Both genera were published in a paper in 1922 by the original author who revised the family at that time. The original...
19 January 2021 1,920 4 View
Healthcare providers have the duty and commitment to protect patients from harm. One way of achieving this goal is by getting the vaccines. But we also learn that the providers themselves are...
13 January 2021 3,638 4 View
Our study is investigating clinical knowledge in a specific area in healthcare workers at a large metropolitan hospital. Participants will self-select to complete the survey via email invitation...
11 January 2021 7,934 2 View
Business in informality can be caused by factors such as the facilitating business environment, tax rates, forms of consumption by buyers, lack of administrative knowledge, complexity of the...
01 January 2021 7,189 5 View
The presence of SARS-CoV-2 antibodies confers subsequent immunity in most people for at least six months, a study of healthcare workers in Oxford, UK, has...
31 December 2020 8,973 6 View
Hello, My manuscript has been rejected by the editor and reviewers even after the 1st revision. Though I tend to disagree some of the points raised, assume I can't request for another revision...
28 December 2020 6,062 3 View
I am trying to help a friend resolve a problem I have never encountered as an author or as a journal editor. The manuscript was transferred to a journal belonging to Elsevier in December 2018. It...
26 December 2020 9,584 3 View
It's developed by Jay Peters, Carey Nason, and Winston M. Turner as a revised version of Hypermasculinity Index.
24 December 2020 4,450 3 View