Routine isolation and identification of aetiological agents of Superficial Mycoses is of utmost importance in a tropical, humid country densely populated compared with the available land area. For instance, Sri Lanka is ideal with a population of 22.16 million limited to a 65,610 km2 island. Interpretation of results in Mycology needs working experience: how to differentiate pathogens from contaminants depending on the site and the type of sample. Superficial lesions are more prone to contamination with environmental fungal spores. Working experience is essential to increase the accuracy and precision of mycology reports.
Thank you for the answer. Have you ever done any experiment on efficacy of 70% alcohol as a sporicidal disinfectant against fungal spores for instance Aspergillus, Penicillium, Mucor and Rhizopus spores??
Many thanks, Phil, that is very kind of you. You are the only person who answered my query.
Did you publish your articles in Nature Reports?
Well, It means preservation and disinfection go hand in hand in disinfection and sterilization applicable to all laboratories whether a medical or non-medical, handling hazardous biomaterial ?
Alcohol with a concentration of 70% is better for sterilization, because it contains a larger amount of water, which helps it dissolve slowly, and thus achieves a longer period of time for penetrating cells and killing bacteria and germs.
Please read the article attached here. I have been doing surface disinfection for heavily soiled lesions to get rid of contaminants / commensals which may inhibit the growth of pathogens. Working experience is needed to avoid what you speculated that there would be no reason to culture the lesion. I did not come across any incident violating my speculations or postulates when I worked as a Medical Laboratory Technologist at the Department of Mycology, Medical Research Institute, Colombo 08, Sri Lanka, South Asia.
Did you understand? please try to learn from a technologist who is actively doing isolation and identification of aetiological agents of Superficial Mycoses and that would be the best way to understand this.
Adequately cleaning the affected area is the way to disinfect the skin of the targeted area pulsing the vein before blood drawing. Please go through the article attached here and understand how non-disinfection affected the final outcome. The environmental contaminants they reported as other organisms but Phialophora verrucose is a causative agent of chrmoblasto mycoses not Tenia capitis.
Manosha Perera
The article is not relevant.
There was no comparative leg using ethanol. Isolation of Phialophora verrucose (corrected - verrucosa) and Acrophialophora fusipora does not mean they came from the wound - more likely were airborne contaminants encountered in sampling to culture.
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology
- Antibodies