Lakshani Perera

10 Questions 11 Answers 0 Followers

Questions related from Lakshani Perera

What is the best way to plot Ct values for a gene expression in different Samples ?

Hi I want to plot a graph for Ct values from qPCR assay that resulted from a gene expression in different samples.

30 October 2020 1,595 3 View

Can anyone help me with this qRT PCR related problem?

This is my first time doing at quantitative real-time PCR and I'm trying to do the absolute method where we plot the standard and then get the quantification using Unknown Ct value. My unknown...

24 July 2019 478 4 View

How do I amplify a 7.4 KB fragment in mouse genomic DNA ?

I am struggling to amplify an 7.4 kB fragment which I need to confirm my CRISPR KO. I have tried Pfu and Phusion polymerase, used both HF and GC buffers, tried 50 - 70 C temp gradient, DMSO...

28 March 2019 9,864 3 View

How to design best primers to amplify a region in mice genomic DNA?

What is the database I should select under the Primer Pair specificity parameters in NCBI primer design tool?

15 March 2019 7,855 2 View

Can anyone suggest me a method to analyse altered replication dynamics other than DNA fiber analysis?

One of the compounds that we synthesized in our lab can result in replication fork collapse. We have tested for gmma H2AX foci that shows the ds strand breaks. But we need to test for the...

25 March 2017 3,222 0 View

Is there a chance for O-propylalkoxyamine to undergo C-ON bond homolysis under mild conditions (35-40 C )?

Is there a chance for O-propylalkoxyamine to undergo C-ON bond homolysis under mild conditions (35-40 C )? any idea about bond dissociation energy ?

01 August 2016 3,292 3 View

How can I speed up a Mitsunobu reaction?

Reagents are N- hydroxyphthalimide and 3-butynol. reaction condition: THF/ rt

24 July 2015 8,713 16 View

Can anyone suggest a recipe to make an Electroporation buffer to transfect CH12F3 cells ?

I’m having trouble to transfect CH12F3 cells. I tried lipofection using lipofectamine, it didn’t work. We have a biorad gene pulser xcell and I’m thinking to do an Electroporation.

01 January 1970 4,241 3 View

Can anyone help me with this Urea-PAGE gel issue?

When I run my 17mer DNA oligos on PAGE gel I can see a smear above my DNA band. Does anyone know a possible reason or how to minimize this? I have attached a gel image here as well. Thank you

01 January 1970 4,007 3 View

Can anyone suggest me a suitable solvent system for Cl and azide separation where both are linked to n-hydroxyphthalimide with an ethyl group?

Cl and azide linked to n-hydroxyphthalimide

01 January 1970 7,683 1 View