Lakshani Perera

8 Questions 10 Answers 0 Followers

Questions related from Lakshani Perera

What is the best way to plot Ct values for a gene expression in different Samples ?

Hi I want to plot a graph for Ct values from qPCR assay that resulted from a gene expression in different samples.

30 October 2020 1,512 3 View

Can anyone help me with this qRT PCR related problem?

This is my first time doing at quantitative real-time PCR and I'm trying to do the absolute method where we plot the standard and then get the quantification using Unknown Ct value. My unknown...

24 July 2019 444 4 View

How do I amplify a 7.4 KB fragment in mouse genomic DNA ?

I am struggling to amplify an 7.4 kB fragment which I need to confirm my CRISPR KO. I have tried Pfu and Phusion polymerase, used both HF and GC buffers, tried 50 - 70 C temp gradient, DMSO...

28 March 2019 9,807 3 View

How to design best primers to amplify a region in mice genomic DNA?

What is the database I should select under the Primer Pair specificity parameters in NCBI primer design tool?

15 March 2019 7,805 2 View

How can I speed up a Mitsunobu reaction?

Reagents are N- hydroxyphthalimide and 3-butynol. reaction condition: THF/ rt

24 July 2015 8,681 16 View

Can anyone suggest a recipe to make an Electroporation buffer to transfect CH12F3 cells ?

I’m having trouble to transfect CH12F3 cells. I tried lipofection using lipofectamine, it didn’t work. We have a biorad gene pulser xcell and I’m thinking to do an Electroporation.

01 January 1970 4,213 3 View

Can anyone help me with this Urea-PAGE gel issue?

When I run my 17mer DNA oligos on PAGE gel I can see a smear above my DNA band. Does anyone know a possible reason or how to minimize this? I have attached a gel image here as well. Thank you

01 January 1970 3,969 3 View

Can anyone suggest me a suitable solvent system for Cl and azide separation where both are linked to n-hydroxyphthalimide with an ethyl group?

Cl and azide linked to n-hydroxyphthalimide

01 January 1970 7,644 1 View