How to decrease the Kd value of aptamer to nM?

26 April 2021 1 3K Report

I am currently working on aptamer development. My ELONA results showed that the Kd value of aptamer is 40uM.

How to decrease the Kd value of aptamer to nM? is this possible?

What is the suitable/best binding buffer for ELONA? I am using PBS+1.5 mM MgCl2 as a binding buffer. Increasing MgCl2 can give better results?

What is the optimum incubation temperature for aptamer (room temperature or 370C)?

Does aptamer-protein incubation time affect the Kd value?

Samson Soon

Hi. It is very difficult to improve the binding capacity of an aptamer by physical means as the aptamer used will already have an intrinsic set of properties. In our lab, we design the aptamers to reach a high binding attribute before it is synthesised. We do not employ SELEX for our work.

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