Kasturi Selvam

5 Questions 2 Answers 0 Followers

Questions related from Kasturi Selvam

How to avoid background signal in screen printed electrode?

Hi, I am doing aptamer based electrochemical biosensor. First, I cleaned screen printed gold electrode using 0.5M H2SO4. Then, immobilized aptamer followed by blocking using 2mM MCH. I use...

07 July 2024 9,803 1 View

Autodock Vina Grid Spacing is 0.375 A or 1.0 A?

Hello everyone, I am conducting molecular docking using Autodock Vina. The receptor (protein) is huge therefore the grid spacing 0.375 A is not enough (Autodock Tools). So, I also increased the...

23 March 2024 8,257 1 View

What makes high 260/280 ratio for purified protein?

Hi everyone, I have expressed 6x histidine tagged protein(35kDa) and purified using Ni-NTA agarose (Qiagen). The elution buffer contain 20mM of Sodium phosphate, 500mM NaCl and 250mM Imidazole....

21 December 2023 7,050 1 View

Why Lemo21 E.coli did not grow on LB broth after subculture from overnight culture?

Hi everyone, I am currently working on protein expression and purification. Transformation was successful using Lemo21 E.coli and plasmid pRSETA. Single colony was inoculated into 100mL LB broth...

19 November 2023 119 6 View

How to decrease the Kd value of aptamer to nM?

I am currently working on aptamer development. My ELONA results showed that the Kd value of aptamer is 40uM. How to decrease the Kd value of aptamer to nM? is this possible? What is the...

26 April 2021 3,153 1 View