How can I simultaneously recognize unknown patterns and variables from a given data set?

20 January 2021 6,385 3 View

What is the difference between "pesticides" and "insecticides"? Are they same?

What is the difference between "pesticides" and "insecticides" in English? Are they the equivalent replacement when used in text? I am an ecological researcher who particularly concerns the...

04 January 2021 6,230 37 View

Viscosity of CsPbI2Br inorganic perovskite solution?

I am trying to fabricate the CsPbI2Br-based inorganic PSC. I used the solution with a concentration of 1.2M following some papers, but I found that the viscosity of the solution is really high....

20 October 2020 6,167 2 View

How to extract membrane protein of platelet ?

I would like to extract membrane proteins of platelets, but I can't find more detailed protocols. How should I extract without a commercial kit?

25 September 2020 1,165 3 View

Using fixed independent variable across time period?

Hello I'm working on a research on The effect of certain demographic information, Say population density, on people's response at different stage of Covid 19 pandemic. Now the problem is, the...

11 May 2020 7,462 4 View

Is actin or any other housekeeping proteins a suitable loading control in WB in the sequential extraction samples?

Hello guys, I am recently doing WB to detect soluble and insoluble species of a protein using sequential extraction. I have a question about the loading control. Generally, we use...

09 October 2018 6,837 2 View

Why the ZnO I grinded photodegrade slower than bulk?

These days, I do dye degradation experiment. I use mortar and pestle to grind bulk ZnO 10 min, the powders turn yellow. Then use that powder to do dye degradation, why it degrades slower than...

05 August 2018 1,769 4 View

Genomic contamination in RNA extraction with PicoPure RNA extraction kit and Purelink DNase set?

I am working on a project to measure the gene expression on rare cells with a white blood cell background by using the digital PCR technique. The first thing is I need to make sure the tested gene...

21 September 2017 2,012 1 View

What is this contaminant in my cell culture?

11 October 2016 2,530 3 View

How can I do to make the fiber still exist in the high-energy beam?

08 October 2016 8,720 8 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

Help with culturing monolayer of human brain microvascular endothelial cells (hBMEC)?

I've been having a hard time forming an endothelial monolayer with good tight junctions. The person who trained me on the culture of endothelial cells worked mostly with mouse BMEC, and he used...

22 February 2021 8,253 1 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

Can anyone suggest a software for running a plant tissue culture lab?

A software which can help to design the experiment, store data, update new data, set subculture reminders, barcoding etc. Like Invitrosoft.

17 February 2021 3,015 5 View

3T3-L1 cells, is this a bacterial contamination or a cross contamination with different cell line? or something else?

Hi, I am experiencing some difficulties with 3T3-L1 cells culturing recently. I am working with 3T3-L1 cells for almost 4 years now and have seen bacterial contaminations/ unhealthy cells and...

13 February 2021 4,040 3 View

What is the method of removing RNase contamination from a filter paper surface?

I am working on a biosensing project on paper substrate, however, it is found that the RNase contamination occurs on the filter paper. Might I ask is there any method to clean the paper surface...

12 February 2021 3,926 3 View