How can I simultaneously recognize unknown patterns and variables from a given data set?

20 January 2021 6,385 3 View

What is the difference between "pesticides" and "insecticides"? Are they same?

What is the difference between "pesticides" and "insecticides" in English? Are they the equivalent replacement when used in text? I am an ecological researcher who particularly concerns the...

04 January 2021 6,230 37 View

Viscosity of CsPbI2Br inorganic perovskite solution?

I am trying to fabricate the CsPbI2Br-based inorganic PSC. I used the solution with a concentration of 1.2M following some papers, but I found that the viscosity of the solution is really high....

20 October 2020 6,167 2 View

How to extract membrane protein of platelet ?

I would like to extract membrane proteins of platelets, but I can't find more detailed protocols. How should I extract without a commercial kit?

25 September 2020 1,165 3 View

How can i control the hourglass in lsdyna for ceramic materials?

I am working on ballistic simulation. The first issue is how can i control hourglass for ceramic materials. Because if hourglass exceeded 10% of internal energy, the simulation will be...

11 May 2020 7,494 2 View

Using fixed independent variable across time period?

Hello I'm working on a research on The effect of certain demographic information, Say population density, on people's response at different stage of Covid 19 pandemic. Now the problem is, the...

11 May 2020 7,462 4 View

Is actin or any other housekeeping proteins a suitable loading control in WB in the sequential extraction samples?

Hello guys, I am recently doing WB to detect soluble and insoluble species of a protein using sequential extraction. I have a question about the loading control. Generally, we use...

09 October 2018 6,837 2 View

Why the ZnO I grinded photodegrade slower than bulk?

These days, I do dye degradation experiment. I use mortar and pestle to grind bulk ZnO 10 min, the powders turn yellow. Then use that powder to do dye degradation, why it degrades slower than...

05 August 2018 1,769 4 View

Genomic contamination in RNA extraction with PicoPure RNA extraction kit and Purelink DNase set?

I am working on a project to measure the gene expression on rare cells with a white blood cell background by using the digital PCR technique. The first thing is I need to make sure the tested gene...

21 September 2017 2,012 1 View

How can I idetify the organisms from nitrifying, ammonifying and denitrifying bacteria in lacustrine sedimentary organic matter?

05 November 2016 5,180 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

Best protocol for immunogold using araldite resin ?

Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...

14 February 2021 4,959 1 View

How to improve the adhesion of epoxy coating on steel substrate? Which factor affects?

I am facing a problem regarding a de-adhesion problem with the coating. A brief description of my methodology is given below. I am using mild or Low Carbon steel as a substrate and I polish the...

14 February 2021 2,167 4 View

Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

FLAG purification, elution doesn't have protein?

I am purifying a heterotetramer, with one subunit having a 1D4 tag and the other having a FLAG tag. I purified from Sf9 with HBS pH7.3 and 0.002%LMNG first with Anti-1D4 resin and got an adequate...

02 February 2021 3,860 1 View

How long can synthesized peptides using SPPS be stored? Storage Conditions?

I have been synthesizing peptides on resin beads. I am wondering what best storage method is there to store the beads after synthesis before cleaving. And how long can they be stored in that...

02 February 2021 5,636 5 View

The peptide synthesized with CTC resin have mass more (+68 Da) than the desired mass of the synthesized peptide. What could be the reason?

I am synthesizing 7 amino acid long peptide sequence with CTC resin, 50oC coupling for 10 min. On cleavage (TFA/TIPS/Water 95:2.5:2.5) I got peptide with good yield. However, It has 68 Da more...

30 January 2021 8,483 3 View

What are the governing equations for "Impact Classifier Mill" in Powder Coating Industry?

In Powder Coating Industry, Is there any equation that relates physical properties of chips (granules of thermosetting polymer, i.e, extrudate that has been cooled and crushed) such as dimensions...

26 January 2021 1,077 3 View