I Am trying to culture lactobacillus and Candida together. I am having difficulty because lactobacillus likes to grow anaerobically whereas candida prefers aerobic conditions. If anyone has any suggestions on how to solve this dilemma, growing them together, can you please let me know? I am using MRS and YPD media 50%:50%
Hi Umair, C. albicans can create an anoxic microenvironment that supports anaerobic bacterial growth. We demonstrated that in the case of Porphyromonas gingivalis, but others have been shown to other species (check Fox et al. 2014 doi:10.1016/j.cub.2014.08.057). In addition, C. albicans can grow in anaerobic conditions too, I think that you need to draw well your scientific question, perhaps anoxia is more interesting for you. To assess that, the best method is CFU of the heterotypic biofilm, and check separately both species.
It is normal to grow Candida albicans cells in 5% CO2 together with human cells, such as macrophages or those in reconstituted epithelia. Hence, there should be no problem for both to live together.
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