Hi everyone,
I am working on a candidate of a sulfate-reducing bacteria, which showed some traits of sulfate reduction, including making black particle, bad odor. The bacteria was grown on marine broth in anaerobic condition, which contain both Fe2+ and SO4(2-). So we hypothesized this bacteria is a sulfate-reducing bacteria.
One point is that this bacteria belongs to Bacteroidetes phylum, which did not have any member showing the sulfate reduction.
For further evidence, I test the sulfate reduction by measurement the ability to make precipitate of broth culture with BaCl2 solution, which prepare as the method in this paper:
Article Toxicity of hydrogen sulfide toward sulfate-reducing bacteri...
When I measure the OD, I used BaCl2 solution as the blank, and the mixture of broth culture at starting culture with BaCl2 solution as the staring point. However, when I daily measure the OD, the OD was not much changed, though the black particle appeared more abundance day by day.
So could someone can suggest me any other resolution to determine the bacteria is an sulfate reducing bacteria, please?
Thank you in advanced!
Sulfate-reducing bacteria (SRB) are a heterogeneous group of microorganisms which is widespread in anaerobic places where sulfate-containing compounds are present. These microorganisms use sulfate ions, which are reduced to hydrogen sulfide in the process called “dissimilatory sulfate reducing” or “sulfate respiration.” In this process, sulfate is a terminal electron acceptor For the implementation of dissimilatory sulfate reduction, exogenous electron donors are necessary. Molecular hydrogen is the main electron donor for all SRB, but commonly used electron donors are also lactate, acetate, pyruvate, ethanol, fatty acids, amino acids, dicarboxylic acids, and other organic compounds.
the modified Postgate agar medium of the same composition like liquid should be prepared but in this case adds to the medium additional compounds: Na2SO3 (7.5 g/l) and microbiological agar (12 g/l). Sterilize it by autoclaving like Postgate liquid medium. Sodium sulfite in high concentration in medium inhibits most of intestinal species of Enterobacteriaceae family, including Bacteroides, Pseudomonas, and Clostridium, Escherichia, which can be satellites of SRB. The species of SRB are resisted to sulfite ions and can be used as an alternative electron acceptor in the process of dissimilatory sulfate reduction since they have sulfite reductase activity.
- Badges
- Science method