I used a kit to clean up a PCR and purify my DNA. The DNA Needs to be at a higher concentration for me to use in electroporation experiments. How do I concentrate my DNA? Should I be worried about concentrating the salts in the buffer? I would assume it is not possible to run again in the kit. In the future, should I elute in water instead of the buffer provided with the kit?
When selecting your elution buffer, it is important to consider the requirements of your desired downstream processes. Eluting and storing the DNA in TE buffer, for example, is helpful as long as the EDTA does not impact your chosen downstream applications. EDTA chelates, or binds, magnesium present in the purified DNA and can help inhibit possible contaminating nuclease activity. If EDTA is a concern, we recommend storing DNA in a buffered solution, as the acidic nature of DNA can lead to auto-hydrolysis. Alternatively, you can use TE-4 buffer, which is 10mm Tris-HCl, 0.1mm EDTA (pH 8.0).
you can provide a condition for evaporation of water...
not in a high temperature
Hi. I suggest the folloeing website for you:
https://blog.dnagenotek.com/blogdnagenotekcom/bid/35326/7-simple-steps-to-maximize-dna-yield-with-oragene-dna
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