Can the same primer pair , say for instance ITS 1 and ITS 4 generate different molecular weight amplicon after PCR for different fungal species?

Say Fungus A gives 500 bp and Fungus B gives 550 bp is it an absurd result?

29 December 2020 881 3 View

What is the best way to conduct meta analysis ?

24 November 2020 1,318 8 View

Previously some data I have uploaded in mendeley data base and it provide DOI number. Can I published same data with another journal.Please suggest?

Previously some data I have uploaded in Mendeley database and it provides DOI number. Can I publish the same data with another journal ? Please suggest. Is there any problem of plagiarism with...

27 October 2020 2,801 6 View

If a certain paper cited is written language other than English, does its interpretation in another authors work become the baseline for it?

A paper not written in English , does it need to be read in its original form before being cited by anyone in the paper that they are writing . Or it is enough to view its interpretation in...

11 October 2020 4,280 8 View

If a system is stable in each of the operating regions, can it be said that the system is stable for the entire operating region?

If an operating region is divided into a number of sectors and if it is shown that the system is stable in equilibrium point corresponding to each of the sectors, can it be said that the system is...

21 March 2020 8,816 6 View

How to incorporate random uncertainty within the A-matrix represented by a gain block in Simulink?

I have a Simulink gain block that houses the A- matrix of the plant and I want to add randomness into the parameters of A-matrix. How do I achieve that in Simulink?

05 December 2019 6,127 7 View

How can one characterize anti biofilm mechanism of any phytochemical at molecular level ?

I have seen the antibiofilm effect of a phytochemical. It disrupts EPS but I want to know the mechanism of that agent at the genetic and molecular level . What experiments should I employ?

19 November 2019 1,139 5 View

What non-polarizing beam splitter should I use to split reflected light, but not the incoming one?

I am making a polar moke setup and need a non-polarising beam splitter which splits the reflected light but not the incoming one. I would be grateful for any suggestion regarding model number/...

14 November 2019 5,868 3 View

Is it okay to use PCA regression on a time series dataset?

I have a dataset of a binary response variable and 22 predictor variables that have multicollinearity. My goal is to make a predictive model. The data was sampled every 30 seconds over three days....

08 October 2019 5,746 2 View

How to do time series analysis with one/few missing data points?

I have three datasets given to me of images of a conveyor belt taken every 30 seconds over 3 days, but one image was removed/lost in one of them and a few (~10-20) in the others. I calculated the...

02 October 2019 3,893 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View