Protein purification for his tagged protein?

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Hamidreza Mollaei

Both His-tagged and cleaved proteins were purified and stored in a buffer containing 500 mM NaCl and 10 mM Tris-HCl at pH 7.5.

Omar Gonzalez-Ortega

pI is irrelevant for imac, the pH and salt concentration of the buffers are the main factors. You just need to be one unit above the pKa of the imidazole group of the histidyl residues. pH is regularly used at 7.0 with at least 500 mM of NaCl.