Dear all, I want to clone a 5kb promoter of a mouse gene. Finally I want to insert this promoter into a gene reporter like luciferase to determine gene regulation.

But now I even have no idea where to start. Should I extract DNA like regular genotyping? Then design several pairs of primers to PCR several fragments of the promoter? Should I ligate each PCR product to be a whole promoter? Which vector should I choose to hold this big promoter? How accurate the PCR will be?

I am reading many protocols now but still cannot figure out. Any input would be greatly appreciated! Thanks a lot in advance!!!