Xiuli Zhao

5 Questions 16 Answers 0 Followers

Questions related from Xiuli Zhao

Anyone has experience with Qsep fragment analyzer?

Hi, I am looking for a fragment analyzer for QC of RNA and DNA. According to Bioptic web (https://www.bioptic.com.tw/applications/laboratory-solution/high-resolution), their instrument can resolve...

23 November 2022 2,162 0 View

Why DNA ladder but not RNA samples disappeared in bleach denatured gel?

I ran my RNA samples in bleach gel with dsDNA ladder on the same gel (Bleach gel: a simple agarose gel for analyzing RNA quality - PubMed (nih.gov)). However, my DNA ladder, even triple amount...

19 August 2022 4,484 0 View

How to clone the promoter region of my gene?

Dear all, I want to clone a 5kb promoter of a mouse gene. Finally I want to insert this promoter into a gene reporter like luciferase to determine gene regulation. But now I even have no idea...

25 May 2016 5,027 1 View

How much DNA should be used per IP in ChIP assay?

Do you know the amount of chromatin needed per IP for ChIP? Abcam said 25ug of DNA per IP, but I want to try to use much less than that. Do you have suggestions?

25 January 2013 7,773 8 View

Why are there blobs in my sheared DNA bands?

I have some problems with my sheared DNA now. I hope you can help me. Attached are two images for the same DNA gel. The images were taken at different times. One is at 1:00pm and the other is at...

07 January 2013 9,734 15 View